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An examination of the up-regulation of a circadian gene dbp in the liver tissues of HBsAg positive transgenic mice |
LI Xiao-xiao1; REN Jun1; WANG Long2; MA Zhang-mei1; WANG Zhu-gang2; XIE You-hua1.3; Wen Yu-mei1.3 |
| 1. Ministry of Education/Ministry of Health Key laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan university, Shanghai 200032, China; 2. Shanghai research center for model organisms, Shanghai 201210, China; 3. Institute of biomedical sciences, Fudan University, Shanghai 200032, China. |
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Abstract Objective To verify the changes of a circadian gene dbp ( coding for the D site albumin promoter binding protein) in liver tissues of HBsAg positive transgenic mice. Methods Real-time fluorescent quantitative PCR was used to compare the transcriptional levels of dbp in male and female HBsAg positive transgenic mice versus their controls. Individual transgenic mouse and mice at different time points were used to study the regulation of dbp. Results In the liver tissues from both #59 and #10 lineages of HBsAg positive transgenic mice, dbp was up-regulated, compared to their control counterparts, but there was marked individual variability. Preliminary results showed that in HBsAg negative female mice, the transcriptional level of dbp was higher than that in male mice. This difference was more marked in HBsAg positive female transgenic mice. At 8:00 and 2:00, dbp was up-regulated in HBsAg positive transgenic mice, compared to the controls. However, at 8:00 and 2:00, the dbp transcriptional levels were similar between HBsAg positive transgenic mice and the controls. Conclusions For the first time it is reported that dbp was up-regulated in HBsAg positive transgenic mice, which provided evidence that HBV protein expression could be associated with changes in a circadian gene. Further studies are needed to confirm whether this up-regulation can also be found in patients, and clarify the implications of this observation.
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Received: 01 January 1900
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