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| Antigenicity and conservation of a novel protein belonging to OmpA family in Leptospira interrogans |
| LIN Wei;YANG Hong-liang;YANG Yang;HU Bao-yu;DONG Ke;TAN Li-zhi;GUO Xiao-kui |
| Department of Medical Microbiology and Parasitology, Shanghai Jiao Tong University,Shanghai 200025,China |
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Abstract Objective To clone and express a novel OmpA gene (LAO301) from Leptospira interrogans , serogroup ictemhaemorrhageae serovar from the Lai strain and to analyze the antigenicity and conservation of this protein from fifteen different strains belonging to 15 prevalent semgmups of L. interrogans from China. Methods The characteristics of LA0301 were predicted by bioinformatics software.The prokaryotic expression system of LA0301 was constructed by routine molecular biological techniques and expression of the recombinant protein was identified by SDS-PAGE and Western blot after induction with IP1'G. Western blot was used to identify the antigenicity and the conservation of the expressed LA030I in fifteen strains from 15 prevalent serogroups in China.The LA0301 antibody from rabbit sera infected with whole leptospires from the Lai strain was identified by Western blot and F.IISA. Results LA0301 is a novel gene belonging to the OmpA family of L. interrogans . The prokaryotic expression system of LAO301 was constructed successfully. The anti-recombinant protein serum titer in BALB/c mouse was 1: 32, 000. The LA0301 protein was detected in all tested strains of L. interrogans . Rabbit serum infected with L. interrogans from the Lai strain was positive for recombinant protein antibody as detected by Western blot and Fl ISA. Conclusion LAO301, a novel gene belonging to the OmpA family, not only displays good antigenicity and conservation but also can elicit antibody responses in rabbits that are infected with L. interrogans from the Lai strain. Our studies on LA0301 provide a basis for developing novel vaccine candidate of leptospira.
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Received: 01 January 1900
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Corresponding Authors:
YANG Hong-liang
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