酶联免疫吸附试验夹心法检测解脲脲原体方法的建立

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Abstract Objective To establish an antibody-sandwich enzyme linked immunosorbent assay (ELISA) for the detection of Ureaplasm urealyticum (UU) . Methods We used an antibody sandwich ELISA to detect UU and compared the results with cell culture method. Results The rate of sensitivity of the ELISA was 92.6% and the rate of specificity was 97.4% . The lowest detection limitation was 5～10 ng/ml of UU antigen. Conclusion The antibody-sandwich ELISA is a sensitive, convenient and fast method for detection of UU for the screening of large numbers of specimens.

Key words： Ureaplasma urealyticum Cell culture Antibody-sandwich ELISA

Received: 01 January 1900

Corresponding Authors: ZOU Xian-biao

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	ZOU Xian-biao
	ZHANG Guo-wei

Cite this article:

ZOU Xian-biao,ZHANG Guo-wei.

An antibody-sandwich enzyme linked immunosorbent assay to detect Ureaplasma urealyticum

[J]. Journal of Microbes and Infections, 2007, 2(4): 209-210 .

URL:

http://jmi.fudan.edu.cn/EN/ OR http://jmi.fudan.edu.cn/EN/Y2007/V2/I4/209