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The application of variable number of tandemrepeats in the microevolution study of Mycobacterium tuberculosis strain of Beijing genotype in Chongming Island, Shanghai |
QIAO Ke1,*; WANG Hui1,*; YANG Chong-Guang1; LUO Tao1; MEI Jian2; GAO Qian1 |
1. Key Laboratory of Medical Molecular Virology of Ministry of Education and Health, Shanghai Medical School, Fudan University, Shanghai 200032, China; 2. Department of Tuberculosis Disease Control, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China |
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Abstract To find the variable number of tandemrepeat ( VNTR) locus combinations that adapt to study the microevolution of Mycobacterium tuberculosis ( M. tuberculosis ) strain of Beijing genotype and the molecular markers to distinguish diff erent sublineages , VNTR and single nucleotide polymorphism( SNP) genotyping were usedto genotype 135 strains fromculture-positive sputumof tuberculosis patients ( collected and preserved by Shanghai Municipal Center f or Disease Control and Prevention) in Chongming Island , Shanghai . Based on the data, the mini mumspanning tree ( MST) was constructed . The results showed that the genotyping inf ormation of 16-locus VNTR best matched the SNP genotyping data, indicating that it was applicable in the microevolution study of M. tuberculosis strain of Beijing genotype in Chongming Island ,
Shanghai . SNP hapl otypes were mainly concentrated in ST10 ( 42. 2%) , ST19 ( 30. 4%) , and ST22 (14.1 %) . Four VNTRloci ( Mtub21 , QUB26 , MI RU16 and QUB4156) may be the molecular markers to distinguish different sublineages of Beijing genotype strains .
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Received: 01 July 2010
Published: 25 December 2010
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Corresponding Authors:
GAO Qian
E-mail: qgao99@yahoo.com
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Cite this article: |
QIAO Ke,YU Hui,YANG Chong-An等. The application of variable number of tandemrepeats in the microevolution study of Mycobacterium tuberculosis strain of Beijing genotype in Chongming Island, Shanghai[J]. Journal of Microbes and Infections, 2010, 5(4): 208-213.
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URL: |
http://jmi.fudan.edu.cn/EN/ OR http://jmi.fudan.edu.cn/EN/Y2010/V5/I4/208 |
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