|
|
|
| The functional unit of twin-arginine translocation system in El Tor Vibrio cholerae |
| ZHU Zhao-Qin1; JING Huai-Qi2; CHEN Hai-Li1; HU Yun-Wen1; KAN Biao2 |
| 1. Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China; 2. Department of Diarrheal Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
|
|
|
|
Abstract The paper aims to determine the minimum functional unit of twin-arginine translocation (Tat) system by analyzing the Tat system gene cluster composition, which was deduced by homology comparison between Tat gene cluster of Escherichia coli (E. coli) K12 and Vibrio cholerae (V. cholerae) N16961. Tat mutant strains were constructed by deleting tat genes of the N16961 reference strain either individually or double genes combined together. Tat transport system minimum functional unit were then analyzed by comparing the phenotypes among Tat mutant strain, complement strain and wild strain. The data showed that the Tat system gene cluster of V. cholerae N16961 consisted of tatA, tatB, tatC and tatA2 , among which, tatA, tatB and tatC located in the chromosomeⅠand tatA2 gene located in the chromosome Ⅱ. The tatB and tatC genes were indispensable for Tat system functions, while tatA2 might play a role similar to tatE in E. coli. The tatB and tatC combined together constitutes the minimum functional unit of V. cholera Tat system. TatA and TatA2 have similar functions and may share the same functional role in the Tat system.
|
|
Received: 10 February 2012
Published: 01 January 2012
|
|
Corresponding Authors:
KAN Biao;HU Yun-Wen
|
|
|
|
|
|
|
