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| Detection of herpes simplex virus infection in umbilical cord blood in 51 neonates |
| REN Peng1, YAO Juan1, JIN Yan2, LI Fa-Jin1, WANG Xiao-Ting1, GE Jun1, JIANG Long-Feng1, ZHAO Lei1, JI Yang1, WANG Ming-Li1 |
| 1. Department of Microbiology, Anhui Medical University, Hefei 230032，China; 2.Hefei Maternity and Child Health Hospital, Hefei 230001, China |
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Abstract The present paper aims to investigate the herpes simplex virus (HSV) infection in neonates. From November 2010 to June 2011, fresh neonatal cord blood was collected from 51 neonates in a Maternity and Children Hospital and transported immediately to our laboratory. After DNA extraction from the samples, nested-polymerase chain reactions (PCR) were performed by using HSV-1 gD gene- and HSV-2 gG gene-specific primers to detect congenital HSV-1 and HSV-2 infections respectively. The PCR products were sequenced toconfirm the HSV infections.. The results indicated that the infection rate of HSV-1 and HSV-2 were 23.53% and 25.49% respectively. The HSV-1/HSV-2 co-infection rate was 7.84%. This study suggests that early diagnosis and treatment of neonatal HSV infection is critical for the prevention of diseases caused by HSV infection.
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Received: 28 November 2011
Published: 01 January 2012
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Corresponding Authors:
WANG Ming-Li
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