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Effect of disinfectants on killing Mycobacterium tuberculosis during surface decontamination in a biosafety level 3 laboratory |
HAN Wen-Dong, SUN Zhi-Ping, DING Yue-Na, QU Di |
Biosafety Level 3 Laboratory, Key laboratory of Medical Molecular Virology, Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China |
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Abstract The application of effective disinfectants during experimental activities of highpathogenic microorganism is crucial for decontamination of laboratory environment to prevent laboratoryacquired infections. In this study, the quantitative germicidal disinfectant validation experiments using membrane filtration suspension were conducted against Mycobacterium tuberculosis (M. tuberculosis) H37Rv. Sodium hypochlorite solution (containing 1 000 mg/L active chlorine), 75% ethanol/water, and Safespore disinfectant killed M. tuberculosis efficiently. When the concentration of ethanol decreased to 37.5%, it was still able to kill M. tuberculosis by prolonging killing time to 10 min. The results suggest that 75% ethanol/water is suitable as conventional disinfectant and sodium hypochlorite solution (containing 1 000 mg/L active chlorine) is suitable under emergency and accident in biosafety level 3 laboratories (BSL3).
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Received: 03 January 2014
Published: 25 October 2015
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Corresponding Authors:
QU Di
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