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| Anti-human immunodeficiency virus type 1 activity of Scirpusin A and Scirpusin B in vitro |
| ZHU Jing-Yu1,2,3，YANG Guo-Xun4, MENG Zhe-Feng1,2,3，ZHANG Xiao-Yan1,2,3，XU Jian-Qing1,2,3 |
| 1. Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China; 2.Institute of Biomedical Sciences, Fudan University, Shanghai 200032, China; 3. Key Laboratory of Medical Molecular Virology, Ministries of Education and Health, Shanghai Medical College, Fudan university, Shanghai 200032, China; 4. School of Pharmacy, Fudan University, Shanghai 201203, China |
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Abstract Scirpusin A and Scirpusin B, two stilbene dimmers purified from medicinal plants, showed potent anti-viral activity. In this study, human immunodeficiency virus (HIV) envelope and vesicular stomatitis virus G protein (VSV-G)-pseudotyped HIV-1 as well as laboratory-adapted HIV-1 strain IIIB were used to evaluate the effects of these two compounds against HIV-1 in vitro. The results indicated that Scirpusin A and Scirpusin B prevented infection of TZM-bl cell line (susceptible to HIV-1 infection) by pseudotyped HIV-1 and laboratory strain IIIB in vitro. Scirpusin B showed a little higher activity than Scirpusin A. The 50% inhibitory concentration (IC50) of the latter was 1.33 μmol/L while the IC50 of the former was 4.77 μmol/L. Scirpusin A and Scirpusin B also showed similar effects on inhibiting VSV-G pseudotyped HIV-1 in TZM-bl cells, suggesting these two compounds can affect the cellular replication process of HIV-1. The preliminary study indicates that Scirpusin A could play antiviral roles after virus entering cell. The detailed mechanism is still a long way to go.
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Received: 01 November 2013
Published: 25 September 2014
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Corresponding Authors:
MENG Zhe-Feng
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