微生物与感染
Jan. 8, 2025   Home |  | About Journal | Editorial Board | Instruction | Publication Ethics | Subscriptions | Contacts Us | CHINESE
JOURNAL OF MICROBES AND INFECTIONS
Article Current Issue| Next Issue| Archive| Adv Search |
Cloning and expression of Zaire Ebola virus glycoprotein in prokaryotic and eukaryotic cells
ZHANG Zhen-Qing1,2, ZHANG Shuang3, HUANG Yi1, ZHANG Bo1, HU Xiao-Min1, YUAN Zhi-Ming1
1. Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; 2. University of Chinese Academy of Sciences, Beijing 100039, China; 3. University of Hubei, Wuhan 430062, China

Download: PDF (1946 KB)   HTML (0)
Export: BibTeX | EndNote (RIS)      
Abstract  Ebola virus belongs to filoviridae. Zaire Ebola virus has a high mortality rate up to 90%. It is transmitted through blood, secretions, organs or other body fluids of infected people or animals. Unfortunately, there are still no approved drugs and vaccines for Ebola virus infection. Considering the increasing commercial and personal communication with countries in Africa, the possibility of Ebola virus invading into China by infected animals and human highlights the necessity for the development of effective vaccines. In this study, a series of glycoproteins (GPs) were expressed by using pET28a() and pcDNA3.1() in prokaryotic and eukaryotic cells, respectively. The results showed that GP1(33­313 aa), GP1(190­313 aa) and nonstructural secretory glycoprotein (sGP) were expressed in Escherichia coli BL21(DE3)in inclusion bodies, while no GP2(502­632 aa) expression could be detected under the same condition. The edited GP, GP1 and GP2 were expressed in HEK293T cells, but not in BHK21 cells. The data provide some clues for the development of antiviral serum and effective drugs for the treatment of Ebola virus infection.

Key wordsEbola virus      Glycoprotein      Cloning      Expression      Antibody     
Received: 15 September 2014      Published: 25 December 2014
Corresponding Authors: YUAN Zhi-Ming   
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
ZHANG Zhen-Qing1
2
ZHANG Shuang3
HUANG Yi1
ZHANG Bo1
HU Xiao-Min1
YUAN Zhi-Ming1
Cite this article:   
ZHANG Zhen-Qing1,2,ZHANG Shuang3等. Cloning and expression of Zaire Ebola virus glycoprotein in prokaryotic and eukaryotic cells[J]. JOURNAL OF MICROBES AND INFECTIONS, 2014, 9(4): 210-216.
URL:  
http://jmi.fudan.edu.cn/EN/      OR     http://jmi.fudan.edu.cn/EN/Y2014/V9/I4/210
Copyright © 2010  Editorial Board of Chinese Academy of Medical Sciences (CAMS) and the Peking Union Medical College (PUMC)
Add:Editorial office of Acta Academiae Medicinae Sinicae , No.9 Dongdansantiao, Beijing PRC(100730)
Fax:010-65133074 E-mail:actacams@263.net.cn
Supported by:Beijing Magtech