Because of the operation of highly pathogenic microorganisms in special laboratory environment, psychological status of the staff in biosafety level 3 (BSL­3) laboratory is the influence factor of laboratory biosafety. In the present paper, the work satisfaction was set as the evaluation indicator of early psychological health. The staff from six BSL­3 laboratories in 5 provinces (Shanghai, Zhejiang, Jiangsu, Fujian and Hubei) were surveyed. The relationship between work satisfaction and job stress was analyzed. The satisfaction rate was 32.2% in the laboratories. Job stress was negatively correlated with work satisfaction. The stressor “social support” in job demand­control (JDC) model was the positive factor of work satisfaction, and the odds ratio (OR) was 4.60 (95% CI 1.25­16.97) in high social support group. The stressors “over­commitment” in effort­reward imbalance (ERI) model was the negative factor of work satisfaction, and the OR was 0.03 (95% CI 0.00­0.28) in high over­commitment group. The results suggest that job stress reduction can raise work satisfaction of BSL­3 laboratory staff by taking measures such as strengthening social support and releasing over­commitment.