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| Effects of T131N/M133T substitutions in hepatitis B virus surface antigen on glycosylation and antigenicity |
| KANG Yao-yue1, GUO Hong-ying2, ZHANG -Yongmei1, ZHANG -Jiming1 |
| 1. Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, 200040, China; 2. Shanghai Public Health Clinic Center, Shanghai 200083, China |
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Abstract To study the impact of T131N/M133T substitutions in hepatitis B virus surface antigen (HBsAg), the proteins expressed in Huh7 and HeLa cells were subjected to Western blotting, enzyme－linked immunosorbent assay (ELISA) and immunofluorescence (IF) staining. The results showed that T131N/M133T substitutions created a new site for N－glycosylation. The detected level of T131N/M133T was significantly low, indicating a potential negative impact on stability and antigenicity of HBsAg.
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Received: 15 January 2015
Published: 25 April 2015
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Corresponding Authors:
KANG Yao-yue
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