|
|
|
| Endoplasmic reticulum stress induced by human rhinovirus 16 2B protein |
| CHI Miao-Miao, SONG Juan, SONG Qin-Qin, YU Jie, LUO Xiao-Nuan, ZHANG Lu, WANG Xin-Ling, HAN Jun |
| State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention,China Center for Diseases Control and Prevention, Beijing 102206, China |
|
|
|
|
Abstract To investigate whether the non-structural protein 2B of rhinovirus 16 induces endoplasmic reticulum stress (ERS) and apoptosis, BHK-21 cells were transfected with a plasmid p2B-GFP expressing rhinovirus 16 2B protein. Biomarkers of ERS and apoptosis were detected respectively. The results showed that 2B protein was localized to the ER apparatus. 2B protein increased the expressions of ERS marker proteins Grp78 and CHOP and the transcriptional activity of activating transcription factor 6 (ATF6). We also found 2B protein induced nuclear condensation associated with apoptosis and degradation of marker protein PARP in BHK-21 cells. In conclusion, the rhinovirus 16 2B protein induces both ERS and apoptosis.
|
|
Received: 16 February 2015
Published: 25 June 2015
|
|
Corresponding Authors:
HAN Jun
|
|
|
|
|
|
|
