This study aims to isolate lytic bacteriophages from Enterococcus faecalis (E. faecalis)，analyze its biological characteristics and to establish the research basis for a phage­based therapy against Enterococcus infections. A lytic bacteriophage (named φEn­-ZZ8) was isolated from the sewage using double­agar method and its basic biological characteristics were analyzed. The results showed that φEn­-ZZ8 had an icosahedral head of 40 nm in diameter and a filamentous tail of 200 nm in length. The adsorption rate of φEn-­ZZ8 reached 90% in 10 min. One­step growth showed that φEn­-ZZ8 had a short latency period of 15 min, a rise period of 40 min, and a burst size of 150 pfu/cell. The genome of φEn­-ZZ8 was sensitive to HindIII, EcoRV, NdeI, PstI, Xba I and Sac II, and the size of the genome was estimated to be 42 kb. Sodium dodecyl sulfate­polyacrylamide gel electrophoresis (SDS­-PAGE) revealed that at least 14 proteins were observed on the gel, ranging from 11 000 to 130 000. The bacteriophage specifically infected and lysed 30.8% E. faecalis isolates. The φEn­-ZZ8 also exhibited stability to the wide range of pH and temperature. The data suggest that phage φEn-­ZZ8 may be a candidate therapeutic agent to control Enterococcus infections.