|
|
|
| Effect of electroporation on immune response of pFUW-Ag85B-Rv3425 in C57BL/6 mice |
| LIANG Shan-Shan1,2, PEI Jie-Ling3, YANG En-Zhuo2, WANG Hong-Hai4, SHEN Hong-Bo2 |
| 1. School of Life Sciences, Shanghai University, Shanghai 200444, China; 2. Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China; 3. Rizhao No.2 Experimental Middle School, Rizhao 276800, China; 4. School of Life Sciences, Fudan University, Shanghai 200433, China |
|
|
|
|
Abstract A novel DNA vaccine pFUW-Ag85B-Rv3425 based on lentiviral vector pFUW was constructed to improve the transfection efficiency in vivo. The DNA injection combined with electroporation in C57BL/6 mice three times with two-week interval was conducted. After the last immunization, the mice were sacrificed. The spleen lymphocytes were isolated and analyzed by flow cytometry. The expression levels of IgG, IgG1, IgG2a, and interleukin 2 (IL-2) in serum were measured by enzyme-linked immunosorbent assay (ELISA). The results showed that DNA injection in combination with electroporation could induce stronger humoral immunity, more Th1-type immune response and significantly enhance CD8+ T cell and CD27+ B cell proliferation. It is indicated that DNA vaccine in combination with electroporation can be taken as a new therapy strategy against tuberculosis.
|
|
Received: 01 April 2015
Published: 25 October 2015
|
|
Corresponding Authors:
SHEN Hong-Bo
|
|
|
|
|
|
|
