Due to the wide application of broad-spectrum antibiotics and corticosteroids, the use of invasive examination instruments, and the increase of immunocompromised patients, Candida albicans (C. albicans) infection which is most frequently encountered among fungal infections, with high mortality and poor prognosis, is increasing. Thus, early and accurate screening is crucial. Molecular typing is an important method to analyze population structure and epidemic of opportunistic pathogenic fungi, which can effectively identify the community or nosocomial infections, track the source of infection, analyze outbreaks, study population dynamics, differentiate multiple infections and assess micro-evolution. Two types of C. albicans genotyping methods are reviewed in the present paper—genotyping methods based on electrophoresis and typing methods based on sequencing technology. And multilocus sequence typing (MLST) is emphatically expounded as the current best C. albicans genotyping methods.