To investigate the clinical characteristics of diabetic foot (DF) and the distribution of bacteria. The clinical characteristics, clinical outcomes and distribution of main pathogenic bacteria of 210 DF cases in Chengyang People’s Hospital of Qingdao were analyzed by SPSS17.0 software. The results showed that DF morbidity was rising. Tendon necrosis, superficial ulcer and dry and wet mixed wound were tha main manifestations. The superficial ulcer had the highest improvement rate. The infection rate of Gram positive cocci was 56.3%. Staphylococcus aureus was the most common pathogen, accounting for 25.2%. The resistance rates of Staphylococcus aureus to vancomycin, linezolid and moxifloxacin were 100%, 99% and 98%, respectively. The infection rate of Gram negative bacillus was 39.8%. The resistance rates of Escherichia coli and Proteus mirabilis to imipenem, cefoperazone sodium and piperacillin were 99%, 98% and 95%, respectively. The results suggest that the distribution of bacteria in DF ulcers is complex. The risk factors of drug resistance include blood glucose, duration and complexity of wound. This study is helpful to guide rational selection of antibiotics in clinic.