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| In vitro study of antimicrobial effect of photodynamic therapy using 5-aminolevulinic acid-mediated photodynamic treatment against methicillin-resistant Staphylococcus aureus strains from different infectious diseases |
| ZHAO Keqing1, YANG Chen1,2, WU Yang3, GUO Limin1, LIU Huayong3, JI Jian4, CHEN Xiaoying5, ZHENG Chunquan1 |
| 1. Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University; Shanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai 200031, China; 2. Department of Otolaryngology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine,, Shanghai 200035, China; 3. Department of Medical Microbiology and Parasitology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China; 4 . Department of Clinical Laboratory, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai 200031, China; 5. Department of Dermatology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200035, China |
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Abstract In photodynamic therapy (PDT), the reactive oxygen species generated by the activation of photosensitizers by light of an appropriate wavelength, are able to inactivate microbes by damaging various microbial components. Due to its multi-targeting antimicrobial capability, PDT has shown a potential as an effective alternative therapeutic option against methicillin-resistant Staphylococcus aureus (MRSA). In this study, MRSA strains isolated from burn, acute pharyngitis, nasosinusitis and pneumonia patients were subjected to a 5-aminolevulinic acid (ALA) mediated light emitting diode (LED) array in vitro. The results showed that a significant reduction (1.8 log10 cfu) was achieved with an ALA treatment (1 h, 5 mmol/L) followed by 1 h of illumination [(633±10) nm wavelength light source, and a 360 J/cm2 light dose]. MRSA strains collected from different tissues showed a similar sensitivity to the treatment.
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Received: 23 November 2015
Published: 25 August 2016
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Corresponding Authors:
ZHENG Chunquan,ZHAO Keqing
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| Cite this article: |
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ZHAO Keqing1,YANG Chen1.2,WU Yang3等. In vitro study of antimicrobial effect of photodynamic therapy using 5-aminolevulinic acid-mediated photodynamic treatment against methicillin-resistant Staphylococcus aureus strains from different infectious diseases[J]. JOURNAL OF MICROBES AND INFECTIONS, 2016, 11(4): 228-233.
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| URL: |
| http://jmi.fudan.edu.cn/EN/ OR http://jmi.fudan.edu.cn/EN/Y2016/V11/I4/228 |
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2016, Vol. 11 
