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| High throughput analysis of phenotypic differences between H37Ra and H37Rv using Biolog Phenotype MicroArray |
| WANG Yihong，GUO Qinglong，ZHOU Fengzhu，GOU Zongchao，WANG Honghai，ZHANG Xuelian |
| State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China |
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Abstract Tuberculosis, caused by Mycobacterium tuberculosis (M. tuberculosis) H37Rv, is a human disease of major importance worldwide. The avirulent M. tuberculosis H37Ra which is used commonly in the laboratory was derived from H37Rv in 1935, but the mechanism of its virulence attenuation remains unclear. In order to explore its possible mechanism of virulence attenuation, screening for different phenotypes is important. To make a rapid and high throughput phenotypic analysis between H37Ra and H37Rv, as well as catch the different phenotypes related to virulence attenuation, the Biolog Phenotype MicroArray analysis was conducted. In this research, the phenotypes of H37Ra were determined and compared with its virulent counterpart H37Rv. Of 765 substrates surveyed, H37Ra phenotypes were highly similar to that of H37Rv, but there were several differences in carbon utilization, osmosis and pH arrays. These arrays suggested that H37Ra could not utilize mono-methyl succinate and Tween 40 as carbon source in contrast to H37Rv. More interestingly, the differences between H37Ra and H37Rv in the tolerance abilities of hyperosmosis and acid were found, and H37Ra could not tolerate circumstance of pH 5.5 or 3% NaCl, but H37Rv could. These metabolic differences may have a disparate impact on the survival of M. tuberculosis in the hosts and be closely related to the virulence attenuation of H37Ra.
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Received: 24 February 2016
Published: 25 April 2016
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Corresponding Authors:
ZHANG Xuelian
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2016, Vol. 11 
