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Detection of neutralizing antibodies against severe fever with thrombocytopenia syndrome virus with enzyme-linked immunosorbent assay-based endpoint assessment |
LIU Dandan1, JIAO Yongjun2, ZHANG Qianyun1, LI Zhifeng2, QI Xian2, SONG Yongchun1 |
1. School of Life Science, Nanjing University, Nanjing 210093, China; 2. Jiangsu Provincial Center for Disease Prevention and Control, Nanjing 210009, China |
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Abstract An enzyme-linked immunosorbent assay (ELISA) method was developed for the detection of neutralizing antibodies against severe fever with thrombocytopenia syndrome virus (SFTSV). Vero-E6 cells in a 96-well micro-plates were challenged with SFTSV first, and then subjected to the treatment with an anti-nucleocapsid protein (SFTSV-NP) monoclonal antibody at different concentrations. The 50% tissue culture infective dose (TCID50) was calculated with Reed-Muench method. 10 double serum samples collected at two different stages of infection were subjected to the assay. 8 specimens demonstrated higher (>4 times) antibody levels in the recovery phase than that in the acute phase. In conclusion, the established ELISA method is specific, operation friendly and with an application potential for clinical research.
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Received: 17 December 2015
Published: 25 June 2016
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Corresponding Authors:
QI Xian, SONG Yongchun
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Cite this article: |
LIU Dandan1,JIAO Yongjun2,ZHANG Qianyun1等. Detection of neutralizing antibodies against severe fever with thrombocytopenia syndrome virus with enzyme-linked immunosorbent assay-based endpoint assessment[J]. JOURNAL OF MICROBES AND INFECTIONS, 2016, 11(3): 171-175.
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URL: |
http://jmi.fudan.edu.cn/EN/ OR http://jmi.fudan.edu.cn/EN/Y2016/V11/I3/171 |
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