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Application of Ki67 and nuclear acid fluorescent dye co-staining in analysis of cell cycle |
SUN Shuhui |
Department of Medical Microbiology and Parasitology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China |
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Abstract The interaction between pathogenic microbes and host cells is crucial for studies on infections and tumor cell biology. The kinetic studies of cell cycle are of significance for understanding the mechanisms mediated by microbes versus host cells. In this paper, a novel method for cell cycle analysis in mouse splenic cells by Ki67 and 4′,6-diamidino-2-phenylindole (DAPI) or propidium iodide (PI) co-staining is introduced.
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Received: 25 April 2016
Published: 25 August 2016
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Corresponding Authors:
SUN Shuhui
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