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| Construction of BA2380 deletion mutant of Bacillus anthracis strain A16D2 |
| JIA Shuhua1,2, CHEN Nan2,3，WANG Dongshu2, WANG Tiantian2，FENG Erling2, ZHU Li2 , WANG Hengliang2, PENG Qingzhong1, LIU Xiankai2 |
| 1. Jishou University, Jishou 416000, China; 2. Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China; 3. College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034, China |
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Abstract The early results of our laboratory showed that BA2380 protein may be related to the virulence of Bacillus anthracis (B. anthracis). The goal of this study is to knock out the BA2380 gene in B．anthracis A16D2 strain. The downstream and upstream sequences of BA2380 and spectinomycin resistance gene (spc) were obtained with polymerase chain reaction (PCR) respectively and used in the construction of plasmid pK2380usd for gene replacement. pK2380usd was introduced into B. anthracis A16D2 competent cells, and the BA2380 gene deletion was obtained by antibiotic screening and PCR identification. The results laid a foundation for the subsequent exploration of gene function.
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Received: 20 December 2016
Published: 25 June 2017
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Corresponding Authors:
PENG Qingzhong, LIU Xiankai
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2017, Vol. 12 
