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| Comparison of microbiota and dissemination of tetracycline resistant bacteria between chickens from a small farmhouse and a big feedlot |
| CUI Zelin1,2, FENG Tingting3, ZHOU Yuhua2, HUANG Qiujing2, WANG Hanyu2, CHEN Chihua2, CAI Mengxin2, QIN Kaijie2, ZHANG Yan2, DONG Ke2, GUO Xiaokui2 , QIN Jinhong2 |
| 1. Department of Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China; 2. Department of Medical Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 3. Department of Clinical Pharmacy, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China |
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Abstract To analyze the microbial composition and the distribution of tetracycline resistant bacteria in the feces of chickens from small farmhouse and big feedlot, eight chickens from a small farmhouse and ten chickens from a big feedlot were enrolled. Fecal microbiota was analyzed through 454 pyrosequencing of 16S rRNA V3 region. Fourteen reported tetracycline resistance genes were tested by polymerase chain reaction (PCR). The mean Shannon diversity indexes of microbiota in chickens from a small farmhouse and a big feedlot were 5.321±0.590 and 4.398 ±0.440, respectively, with significant difference (Mann-Whitney U test, P＝0.008). Moreover, 69 and 65 tetracycline resistant isolates were randomly selected from chicken feces in small farmhouse and big feedlot, respectively. The species of tetracycline resistant bacteria isolated from chicken feces in the big feedlot were more diverse than those in the small farmhouse. The data concluded the wider dissemination of tetracycline resistant bacteria in the chickens from big feedlot, suggesting that more attentions should be paid to the changes in the microbiota and tetracycline resistant bacteria in the chickens from big feedlot and their influences on human health.
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Received: 22 December 2016
Published: 25 June 2017
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Corresponding Authors:
QIN Jinhong, GUO Xiaokui
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2017, Vol. 12 
