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| Development of a method for rapid detection of 10 high-pathogenic viruses based on reverse transcription-loop-mediated isothermal amplification |
| ZHAO Na1，ZHAO Wang2，LIU Qi3，XIA Han1，SHI Yongxia4，YUAN Zhiming1 |
| 1.Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; 2.Shanghai Cohere Electronics Technology Co. Ltd, Shanghai 201611, China; 3.Department of Environmental Science & Engineering，Fudan University, Shanghai 200433, China; 4.Guangzhou Inspection & Quarantine Technology Center, Guangzhou 510627, China |
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Abstract The present study aims to establish a method for rapid detection of a panel of pathogenic viruses based on reverse transcription-loop-mediated isothermal amplification (RT-LAMP). Primer sets of 10 pathogenic viruses with high specificity were screened. It was suggested that the method was safe, rapid, convenient, and high-throughput in detection of pathogenic viruses. When combined with the new immobilized reaction tubes, the amplification for 10 pathogenic viruses in 1-100 copies could be finished within 45 min. In the assay of 30 serum samples, one rift valley fever virus-positive sample was obtained. A rapid, convenient, low-cost, real-time and high-throughput detection method for common pathogenic viruses was established. The results indicate that RT-LAMP method for 10 high-pathogenic viruses has a good application prospect in rapidly screening suspected patients in areas with high population mobility such as ports.
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Received: 04 January 2018
Published: 25 October 2018
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Corresponding Authors:
YUAN Zhiming
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2018, Vol. 13 
