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| Tandem expression of four avian influenza M2e polypeptides and effects of oral immunization in mice |
| LUO Bin1,3, QIAN Kai1,3, XU Wanxiang2, TANG Haiping2, JI Chaoneng1,3, XIE Yi1,3, GU Shaohua1,3 |
| 1. Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China; 2. National Population and Family Planning Key Laboratory of Contraceptives Drugs and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China; 3. Shanghai Engineering Research Center of Industrial Microorganisms, Shanghai 200438, China |
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Abstract In order to develop a universal oral vaccine for bird flu, four avian influenza virus-specific matrix protein 2 ectodomain (M2e) polypeptides were serially ligated with the mucosal immune adjuvant CTA1-DD, expressed in prokaryotic cells and purified as CTA1DD-AVI4M2e fusion protein. The BALB/c mice were orally immunized with the fusion protein CTA1DD-AVI4M2e. The results showed that the intestinal fluid IgA antibody titer and serum IgG antibody titer in the experimental group were significantly higher than those in the control group. The specific IgG antibody titer in the serum was about 360 times；in the first, second, and third segmented intestinal fluid, the IgA antibody titers were about 360 times, 120 times and 9 720 times, respectively. The results demonstrate that CTA1DD-AVI4M2e has a significant oral immune effect, providing the basis for further studies.
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Received: 17 May 2018
Published: 01 January 2018
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Corresponding Authors:
GU Shaohua
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