微生物与感染
Jan. 8, 2025   Home |  | About Journal | Editorial Board | Instruction | Publication Ethics | Subscriptions | Contacts Us | CHINESE
JOURNAL OF MICROBES AND INFECTIONS  2019, Vol. 14 Issue (5): 267-276    DOI: 10.3969/j.issn.1673-6184.2019.05.002
Original Article Current Issue| Next Issue| Archive| Adv Search |
The charge heterogeneity of murine coronavirus nucleocapsid protein is related to phosphorylation
WEN Rong, WANG Yuyan, YE Rong
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), and Department of Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China

Download: PDF (4276 KB)   HTML (490)
Export: BibTeX | EndNote (RIS)      
Abstract  The nucleocapsid (N) protein is an essential structural protein of the virus and plays an important role in protecting the viral genome and regulating the process of viral replication. The phosphorylation of N protein of mouse hepatitis virus (MHV), has been proved to be associated with viral transcription, replication and assembly. We found that N protein expressed at late stage rather than early stage in neuro-2a cells infected with MHV-A59 showed a significant charge heterogeneity. Isoelectric focusing electrophoresis showed that the charge of N protein was continuously distributed. Treatment with the protein S-palmitoylation inhibitor 2-BP and the proteasome inhibitor MG-132 showed that viral replication levels were not only associated with N protein electronegativity, but also with the PKCα phosphorylation at S657 and the maintenance of some endoplasmic reticulum components involved in protein modification and degradation. These results suggest that phosphorylation is a factor associated to the heterogeneity and stability of the murine coronavirus N protein.
Key wordsMurine coronavirus      Nucleocapsid protein      Electronegativity      PKCα; Phosphorylation     
Received: 24 July 2019      Published: 25 October 2019
Corresponding Authors: YE Rong   
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
WEN Rong
WANG Yuyan
YE Rong
Cite this article:   
WEN Rong,WANG Yuyan,YE Rong. The charge heterogeneity of murine coronavirus nucleocapsid protein is related to phosphorylation[J]. JOURNAL OF MICROBES AND INFECTIONS, 2019, 14(5): 267-276.
URL:  
http://jmi.fudan.edu.cn/EN/10.3969/j.issn.1673-6184.2019.05.002     OR     http://jmi.fudan.edu.cn/EN/Y2019/V14/I5/267
Copyright © 2010  Editorial Board of Chinese Academy of Medical Sciences (CAMS) and the Peking Union Medical College (PUMC)
Add:Editorial office of Acta Academiae Medicinae Sinicae , No.9 Dongdansantiao, Beijing PRC(100730)
Fax:010-65133074 E-mail:actacams@263.net.cn
Supported by:Beijing Magtech