微生物与感染
Jan. 8, 2025   Home |  | About Journal | Editorial Board | Instruction | Publication Ethics | Subscriptions | Contacts Us | CHINESE
JOURNAL OF MICROBES AND INFECTIONS  2020, Vol. 15 Issue (3): 158-165    DOI: 10.3969/j.issn.1673-6184.2020.03.004
Original Article Current Issue| Next Issue| Archive| Adv Search |
Evaluation of simultaneous amplification testing and quantitative reverse transcription-polymerase chain reaction for quantitative detecting serum hepatitis B virus RNA
HUANG Chenlu1, XU Wei1, HU Qiankun1, ZHANG Xiaonan1, LI Qiang1, HUANG Yuxian1,2, CHEN Liang1
1. Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; 2. Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China

Download: PDF (868 KB)   HTML (537)
Export: BibTeX | EndNote (RIS)      
Abstract  The purpose of the current study is to evaluate the correlation and consistency of simultaneous amplification testing (SAT) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for quantitative detecting serum hepatitis B virus RNA. 212 serum samples from Shanghai Public Health Clinical Center including 81 CHB patients with HBV DNA≥100 IU/mL, 76 CHB patients with HBV DNA 100 IU/mL and 55 patients without HBV infection were detected by SAT and qRT-PCR methods. In HBV DNA≥100 IU/mL CHB patients, 95.06% (77/81) samples were positive both in SAT and qRT-PCR method. SAT showed a relevantly good correlation and comparability with qRT-PCR (R2=0.803, CCC=0.882). Bland Altman analysis shows absolute bias was 0.1 log10 copies/mL and relative bias was 0.97%. The paired T test analysis of test results had no difference (t =1.617,P =0.110). In HBV DNA 100 IU/mL CHB patients, 98.68% (75/76) samples were positive in SAT method and 88.16% (67/76) samples were positive in qRT-PCR method. The statistical analysis of test results had difference (P<0.001). SAT showed a relevantly bad correlation and comparability with qRT-PCR (R2=0.326, CCC=0.438). Bland Altman analysis shows absolute bias was 0.5 log10 copies/mL and relative bias was 86%. The paired T test analysis of test results had difference (t =3.654,P<0.001). In 55 patients without HBV infection, all samples were negative in SAT method and qRT-PCR method. Correlation analysis shows strong correlation and consistency between SAT and qRT-PCR for CHB patients with HBV DNA≥100 IU/mL. Difference was found in the measurement of RNA using SAT and qRT-PCR for CHB patients with HBV RNA 100 IU/mL.
Key wordsHepatitis B virus      RNA      Quantitative reverse transcription-polymerase chain reaction      Simultaneous amplification testing     
Received: 28 October 2019      Published: 25 June 2020
ZTFEL: R373.2  
Corresponding Authors: CHEN Liang   
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
HUANG Chenlu1
XU Wei1
HU Qiankun1
ZHANG Xiaonan1
LI Qiang1
HUANG Yuxian1
2
CHEN Liang1
Cite this article:   
HUANG Chenlu1,XU Wei1,HU Qiankun1等. Evaluation of simultaneous amplification testing and quantitative reverse transcription-polymerase chain reaction for quantitative detecting serum hepatitis B virus RNA[J]. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(3): 158-165.
URL:  
http://jmi.fudan.edu.cn/EN/10.3969/j.issn.1673-6184.2020.03.004     OR     http://jmi.fudan.edu.cn/EN/Y2020/V15/I3/158
Copyright © 2010  Editorial Board of Chinese Academy of Medical Sciences (CAMS) and the Peking Union Medical College (PUMC)
Add:Editorial office of Acta Academiae Medicinae Sinicae , No.9 Dongdansantiao, Beijing PRC(100730)
Fax:010-65133074 E-mail:actacams@263.net.cn
Supported by:Beijing Magtech