Evaluation of simultaneous amplification testing and quantitative reverse transcription-polymerase chain reaction for quantitative detecting serum hepatitis B virus RNA

HUANG Chenlu1, XU Wei1, HU Qiankun1, ZHANG Xiaonan1, LI Qiang1, HUANG Yuxian1,2, CHEN Liang1

Journal of Microbes and Infections ›› 2020, Vol. 15 ›› Issue (3) : 158-165.

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Journal of Microbes and Infections ›› 2020, Vol. 15 ›› Issue (3) : 158-165. DOI: 10.3969/j.issn.1673-6184.2020.03.004
Original Article

Evaluation of simultaneous amplification testing and quantitative reverse transcription-polymerase chain reaction for quantitative detecting serum hepatitis B virus RNA

  • HUANG Chenlu1, XU Wei1, HU Qiankun1, ZHANG Xiaonan1, LI Qiang1, HUANG Yuxian1,2, CHEN Liang1
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Abstract

The purpose of the current study is to evaluate the correlation and consistency of simultaneous amplification testing (SAT) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for quantitative detecting serum hepatitis B virus RNA. 212 serum samples from Shanghai Public Health Clinical Center including 81 CHB patients with HBV DNA≥100 IU/mL, 76 CHB patients with HBV DNA 100 IU/mL and 55 patients without HBV infection were detected by SAT and qRT-PCR methods. In HBV DNA≥100 IU/mL CHB patients, 95.06% (77/81) samples were positive both in SAT and qRT-PCR method. SAT showed a relevantly good correlation and comparability with qRT-PCR (R2=0.803, CCC=0.882). Bland Altman analysis shows absolute bias was 0.1 log10 copies/mL and relative bias was 0.97%. The paired T test analysis of test results had no difference (t =1.617,P =0.110). In HBV DNA 100 IU/mL CHB patients, 98.68% (75/76) samples were positive in SAT method and 88.16% (67/76) samples were positive in qRT-PCR method. The statistical analysis of test results had difference (P<0.001). SAT showed a relevantly bad correlation and comparability with qRT-PCR (R2=0.326, CCC=0.438). Bland Altman analysis shows absolute bias was 0.5 log10 copies/mL and relative bias was 86%. The paired T test analysis of test results had difference (t =3.654,P<0.001). In 55 patients without HBV infection, all samples were negative in SAT method and qRT-PCR method. Correlation analysis shows strong correlation and consistency between SAT and qRT-PCR for CHB patients with HBV DNA≥100 IU/mL. Difference was found in the measurement of RNA using SAT and qRT-PCR for CHB patients with HBV RNA 100 IU/mL.

Key words

Hepatitis B virus / RNA / Quantitative reverse transcription-polymerase chain reaction / Simultaneous amplification testing

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HUANG Chenlu1, XU Wei1, HU Qiankun1, ZHANG Xiaonan1, LI Qiang1, HUANG Yuxian1,2, CHEN Liang1. Evaluation of simultaneous amplification testing and quantitative reverse transcription-polymerase chain reaction for quantitative detecting serum hepatitis B virus RNA[J]. Journal of Microbes and Infections. 2020, 15(3): 158-165 https://doi.org/10.3969/j.issn.1673-6184.2020.03.004
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