微生物与感染
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JOURNAL OF MICROBES AND INFECTIONS  2020, Vol. 15 Issue (4): 213-219    DOI: 10.3969/j.issn.1673-6184.2020.04.003
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Receptor-interacting protein kinase 3 plays a role in induction viral antigen specific CD8memory T cell responses against influenza A (H1N1) virus infection in mice
QIN Boyin, WANG Chao, LIU Yang, REN Xiaonan, FANG Zhong, LI Shun, ZHOU Xiaohui
Shanghai Public Health Clinical Center , Fudan University, Shanghai 201508, China

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Abstract  Receptor-interacting protein kinase 3 (RIPK3) is one of the key components of necrotic complex, which mediates the development of programmed cell necrosis. Our previous studies have revealed that the initial response of total influenza antigen-specific CD8T cells is partially dependent on RIPK3, but its role in memory CD8T cell response is unclear yet. In this study, we observed that the frequency of influenza specific CD8T cells and their ability to secrete cytokine IFN-γ and TNF-α in RIPK3 knockout mice were significantly lower than those in wild-type mice 37 days after the initial infection of H1N1 A/Puerto Rico/8/34 strain (PR8) in C57BL/6 mice. After the re-challenging infection of H1N1virus PR8, the frequency of viral specific CD8T cells and their ability to secrete cytokine IFN-γ in RIPK3 knockout mice were still significantly lower than those in wild-type mice. In addition, while the frequency of CD8central memory T cells (TCM) in RIPK3 knockout mice was significantly higher than that in wild-type mice, the proportion of effective memory T cells (TEM) or effective T cells (TEff) was significantly lower than that in wild-type mice. These results indicate that RIPK3 is involved in the regulation of the number and secretion of cytokines of influenza virus specific memory CD8T cells, and affects the ratio of TCM to TEM or TEff in memory CD8T cells, which provide new clues for further exploring the molecular mechanism of virus specific memory CD8T cell response.
Key wordsReceptor-interacting protein kinase 3      Influenza A (H1N1) virus      Memory CD8T cells     
Received: 03 April 2020      Published: 25 August 2020
ZTFEL: Q95-33  
Corresponding Authors: ZHOU Xiaohui   
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QIN Boyin
WANG Chao
LIU Yang
REN Xiaonan
FANG Zhong
LI Shun
ZHOU Xiaohui
Cite this article:   
QIN Boyin,WANG Chao,LIU Yang等. Receptor-interacting protein kinase 3 plays a role in induction viral antigen specific CD8memory T cell responses against influenza A (H1N1) virus infection in mice[J]. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(4): 213-219.
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http://jmi.fudan.edu.cn/EN/10.3969/j.issn.1673-6184.2020.04.003     OR     http://jmi.fudan.edu.cn/EN/Y2020/V15/I4/213
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