Abstract This study analyzed the peripheral blood ISG15mRNA expression level in viral pneumonia patients and bacterial pneumonia patients, and discussed the possible relationship between ISG15 and the pathogenesis of viral pneumonia, providing further clinical evidences for host markers of specific respiratory virus infection. 157 adult community acquired pneumonia (CAP) hospitalized patients’ peripheral blood samples and 40 healthy people’s (healthy controls) peripheral blood samples and their clinical data were collected in this study, including 133 patients with respiratory tract infection and 24 patients with bacterial infection. The ISG15mRNA expression level for all samples were analyzed by Real-time quantitative PCR. We validated the ISG15 mRNA expression by stimulating the PBMC in vitro and analyzing the correlation between viral load and ISG15mRNA levels. The expression level of ISG15 mRNA in the respiratory viral infection group was significantly higher than that in the healthy control group and the bacterial infection group. Receiver operating characteristic (ROC) curves analysis showed that the ISG15 mRNA in peripheral blood had a great potential diagnostic value for respiratory virus infection and bacterial infection in adult CAP patients. In vitro studies showed that there was a positive correlation between ISG15mRNA expression in PBMC and viral replication. The peripheral blood ISG15 mRNA expression level were highly correlated with respiratory virus infection by analyzing the clinical data and in vitro experiments. This study demonstrated that ISG15mRNA in peripheral blood may have an important clinical value in diagnosis of viral pneumonia.
Received: 26 August 2020
Published: 01 January 2021