Abstract Rotavirus infection is the leading cause of acute dehydrated gastroenteritis among children under 5 years old worldwide. Diarrhea occurs in infected children. Even more, dehydration and electrolyte disturbances may appear in severe infections, which endanger the health of children and even life. Nonetheless, viral gastroenteritis is often difficult to differentiate from gastroenteritis caused by enteric bacteria on the basis of clinical presentation. Therefore, laboratory tests are required for adequate specific diagnosis. The Electron Microscope(EM), Polyacrylamide gel Electrophoresis(PAGE), Polymerase Chain Reaction(PCR), Enzyme-Linked Immunosorbent(ELISA) and Gold Immunochromatographic Assay(GICA) are used in detecting rotavirus. Different detecting method suit for different situation. Traditional morphological and immunological methods are substituted by molecular diagnostic method, which have high specificity and sensibility. In this article, we would review the method and advanced technologies of rotavirus detection.