In order to investigate the genotyping, molecular epidemiological characteristics in neonatal and maternal infection and sources of specimens as well as antimicrobial susceptibility of Listeria monocytogenes(Lm) in neonatal and maternal in Nanning, Guangxi Province. The Listeria monocytogenes isolated from the department of neonatology and obstetrics of Maternal and Child Health Hospital of Guangxi zhuang Autonomous Region from 2015 to 2017 were retrospectively reviewed, in vitro susceptibility testing and serological typing were carried out by a kit, and multilocus sequencing (MLST) for analysis of strains. And the clinical characteristics and risk factors analysis of perinatal listeriosis were analyzed.The results showed that the lower incidence of neonatal Lm infection was 0.091 ‰ between 2015 to 2017 in Nanning of Guangxi. The isolated Lm belonged to two stronger pathogenic strains serotypes [4b (83.3%) and 1/2a (16.7%)]. Drug susceptibility test showed that Listeria monocytogenes were 100% sensitive to penicillin, ampicillin, trimethoprim/sulfamethoxazole and meropenem, and no resistant strains have been found. The genotyping obtains two sequence types (ST) by MLST, mainly ST­1 type (83.3%). Four strains of bacteria were isolated from a newborn patient(Case 2) of peripheral blood(Lm2), ear swab(Lm3) and amniotic fluid(Lm4) and cervical secretion(Lm5) of her mother, which were confirmed to be the same clone by antibiotic sensitivity test, serotype and MLST. The Listeria monocytogenes infections in pregnant women and their newborn infants may be derived from the same clone via vertical transmission.All the neonates in the reported cases had symptoms, including fever, pneumonia, Bluish­colored skin, Sepsis and Meningitis.All the pregnant women in the reported cases had atypical and nonspecific clinical symptoms.Clinical attention should be paid to the pathogen examination of Listeria monocytogenes in maternal and neonatal infections, early diagnosis, prevention and reduction of maternal­infant infection caused by Listeria monocytogenes by timely and reasonable antibiotic treatment.