新生儿及产妇感染产单核细胞增生李斯特菌分离株的血清型、耐药性及分子特征研究

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Abstract Abstract : In order to investigate the genotyping, molecular epidemiological characteristics in neonatal and maternal infection and sources of specimens as well as antimicrobial susceptibility of Listeria monocytogenes(Lm) in neonatal and maternal in Nanning, Guangxi Province. The Listeria monocytogenes isolated from the department of neonatology and obstetrics of Maternal and Child Health Hospital of Guangxi zhuang Autonomous Region from 2015 to 2017 were collected, in vitro susceptibility testing and serological typing were carried out by a kit, and multilocus sequencing (MLST) for analysis of strains. The results showed that the lower incidence of neonatal Lm infection was 0.091 ‰ between 2015 to 2017 in Nanning of Guangxi. The isolated Lm belonged to two stronger pathogenic strains serotypes (4b (83.3%) and 1/2a (16.7%)). Drug susceptibility test showed that Listeria monocytogenes were 100% sensitive to penicillin, ampicillin, trimethoprim/sulfamethoxazole and meropenem, and no resistant strains have been found. The genotyping obtains two sequence types (ST) by MLST, mainly ST-1 type (83.3%). Four strains of bacteria were isolated from a newborn patient(Case 2) of peripheral blood(Lm2), ear swab(Lm3) and amniotic fluid(Lm4) and cervical secretion(Lm5) of her mother, which were confirmed to be the same clone by antibiotic sensitivity test, serotype and MLST. The Listeria monocytogenes infections in pregnant women and their newborn infants may be derived from the same clone via vertical transmission.Clinical attention should be paid to the pathogen examination of Listeria monocytogenes in maternal and neonatal infections, early diagnosis, prevention and reduction of maternal-infant infection caused by Listeria monocytogenes by timely and reasonable antibiotic treatment.

Received: 15 May 2020 Published: 01 January 2022

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R174+.6 疾病预防

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http://jmi.fudan.edu.cn/EN/ OR http://jmi.fudan.edu.cn/EN/Y2021/V16/I4/242

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