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Detection of Borna disease virus ( BDV) genome in the brain of BDV-inoculated Wistar rats by in situ hybridization |
LIU Ai-Ping;LI Yu-Jun;LI Xiao-Guang;SONG Wu-Qi;LI Ai-Mei;ZHOU Shu-Ru;ZHANG Feng-Min |
Department of Microbiology,Harbin Medical University, Key Laboratory of Heilongjiang Province for Infection and Immunity, Harbin 150086,China |
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Abstract The aim of the present study was to detect the Borna disease virus ( BDV) genome in the brain of BDV-inoculated Wistar rats by in situ hybridization. The BDV p24 sense probe was labeled with digoxigenin ( DIG) using a DIG RNA labeling kit. The labeling efficiency of the BDV p24 sense probe was conformed by spot test, and the specificity was determined by a dot hybridization assay. The BDV genome was then detected with the probe via in situ hybridization in paraffin brain sections of BDV-inoculated Wistar rats. At the third week post-BDV inoculation, the BDV genome was detected mainly in hippocampus and cortex, with minimal labeling in the thalamus and hypothalamus. Whereas at the sixth week post-BDV inoculation, the BDV genome was not only detected in hippocampus and cortex, but was also increased in thalamus and hypothalamus, suggesting that the BDV-infected region was enlarged following prolonged post-inoculation time, and that in situ hybridization with a BDV p24 sense probe can be used to observe the distribution of BDV in the inoculated Wistar rat brain.
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Received: 19 December 2008
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Corresponding Authors:
Zhang Feng-Min
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