Microneedles, as an efficient transdermal immunization modality, can penetrate the stratum corneum and deliver antigens to the immune cell-rich dermis without damaging the neurovascular system. Among them, dissolving microneedles (dMNs) have attracted much attention due to many advantages such as simple preparation, convenient operation, high drug capacity, and less tip waste. In this review, we mainly summarized the immune effect of dMNs vaccines and the current progress of clinical trials, discussed the issues that exist during the use of dMNs and looked forward to the future research, so as to provide a reference for the research of dMNs vaccination and promote its application.