This paper aimed to identify the differences in the diversity and composition of intestinal fungal flora between the plaque psoriatic patients and the healthy individuals. It obtained the fresh fecal samples from the psoriatic (P) patients and the healthy controls (C) during January 2021 to December 2021 in the dermatology clinic, the Fourth Hospital of Hebei Medical University. Fecal samples were processed and internal transcribed spacer (ITS) was sequenced using the Illumina NovaSeq PE250 platform by UPARSE software. Results showed that although a slight elevation was found in the richness and diversity of the intestinal fungi for the psoriasis group, there was no statistical difference between the two groups by the alpha diversity analysis (P>0.05). A significant separation was detected between P and C groups with the partial least squares discriminant analysis (PLS-DA). The species diversity analysis showed that there were more than 10 species with significant different abundance between two groups. Phaffomycetaceae, Psathyrellaceae, Wickerhamomyces, Papiliotrema_flavescens in group P, and Saccharomycopsis, Saccharomycopsis fibuliger, Cutaneotrichosporon_cyanovorans, Cutaneotrichosporon, Aspergillus_conicus, Penicillium sumatraense, Mrakia blollopis, Hyphopichia, Talaromyces_duclauxii in the group C, were found with higher abundance, respectively. The composition of intestinal fungal flora in the plaque psoriatic patients is significantly different compared with the healthy controls.