Cloning and sequence analysis of fbps gene of Streptococcus suis type2

SUN Li-yun; FAN Hong-jie; LU Cheng-ping

PDF(566 KB)

Journal of Microbes and Infections ›› 2006, Vol. 1 ›› Issue (1) : 28-31.

论著

Cloning and sequence analysis of fbps gene of Streptococcus suis type2

SUN Li-yun; FAN Hong-jie; LU Cheng-ping

Author information +

History +

Abstract

Objective To define whether Streptococcus suis type 2 Jiangsu isolate HA9801 prassesses, fbps gene[encoding fibronectin-and fibrinogen-binding protein(FBPS)] and to study its sequence. Methods fbps gene was amplified from genomic DNA of Streptococcus suis type 2 Jiangsu isolate HA9801 by polymerase chain reaction (PCR). PCR product was cloned into the cloning vector pMDT-18. The recombinant fragment of 1 938bp was verified by restriction endonuclease analysis and sequencing. Results The fbps gene nucleotide sequence of Jiangsu isolate was more than 99.99% identical to that of SS2 Netherland strain as reported by Greeff and to that of ATCC strain. FBPS displayed 76.0%, 73.0%, 72.4%, 70.1% and 68.8% identity with FbpA of S. gordonii (CAA4628.2), putative fibronectin/ fibrinogen-binding protein of S. mutan (AAN59108.1), PavA of S. pneumoniae, (AAK75087.1), adherence and virulence protein A of S. agalactiae 2603V/R(AAN00072.1) and FBP54 of S. pyogenes(AAA57236),respectively. Conclusion Based on stucture analysis,FBPS was predicted as an adhesin without anchoring component.