Objective To explore the characteristic and reason for indeterminate HIV Western Blot(WB),the limitation of WB and the possible amendatory measures.Methods Summing up and analysis the distribution of the indeterminate HIV WB among the tested people,the laboratory test results,the follow-up and the final HIV antibody outcome.Results The relative healthy people including the people of voluntary counseling and testing,the blood donors and pregnant women make up 50% of the indeterminate HIV WB,the follow-up for the indeterminate HIV WB is difficult and the there are few people of the indeterminate HIV WB have HIV antibody test again.There is false-positive in WB test,especially the false-positive of P24 is serious.Conclusion The indeterminate HIV WB is related to the false positive of WB test,measures should be taken to reduce the indeterminate HIV WB and to interpret the result accurately.