Objective To develop a rapid and sensitive assay system to detect the seven virulence factors of Streptococcus suis,using two seqarate multiplex PCR assays.Methods The seven virulence factors were detected based on the gene sequences of mrp,epf,sly gdh,gapdh,orf2 and fbps.PCR primer pairs were produced for each of the 7 factors and the assay method,was developed and optimized.These genes were tested in 29 bacterial isolates by the multiplex assay.Results All PCR products were analyzed by electrophoresis on 2% agarose gels.Detection of all 29 Streptococcus suis strains was confirmed by bacteriological examination.The assay was validated by the positive and negative controls.Conclusion The results demonstrate that the multiplex PCR assay is a highly specific and sensitive diagnostic tool for the detection of seven virulence factors of Streptococcus suis. if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}