An antibody-sandwich enzyme linked immunosorbent assay to detect Ureaplasma urealyticum

ZOU Xian-biao; ZHANG Guo-wei

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Journal of Microbes and Infections ›› 2007, Vol. 2 ›› Issue (4) : 209-210.

论著

An antibody-sandwich enzyme linked immunosorbent assay to detect Ureaplasma urealyticum

ZOU Xian-biao;ZHANG Guo-wei

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Abstract

Objective To establish an antibody-sandwich enzyme linked immunosorbent assay (ELISA) for the detection of Ureaplasm urealyticum (UU) . Methods We used an antibody sandwich ELISA to detect UU and compared the results with cell culture method. Results The rate of sensitivity of the ELISA was 92.6% and the rate of specificity was 97.4% . The lowest detection limitation was 5～10 ng/ml of UU antigen. Conclusion The antibody-sandwich ELISA is a sensitive, convenient and fast method for detection of UU for the screening of large numbers of specimens.