Subcellular location of Rv2629 in M. tuberculosis and its transformed version into M. smegmatis using the pMV261 plasmid

WANG Qing-zhong1; ZHANG Lu1; XU Ying1; CHEN Jia-zhen1; ZHU Bing-dong1; QIE Ya-qing1; WANG Jiu-ling1

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Journal of Microbes and Infections ›› 2008, Vol. 3 ›› Issue (2) : 90-93.

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Subcellular location of Rv2629 in M. tuberculosis and its transformed version into M. smegmatis using the pMV261 plasmid

WANG Qing-zhong1,2; ZHANG Lu1; XU Ying1; CHEN Jia-zhen1; ZHU Bing-dong1; QIE Ya-qing1; WANG Jiu-ling1; WANG Hong-hai1

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Abstract

Objective To detect the subcellular location of Rv2629 in Mycobactrium tuberculosis, and the drug susceptibility testing of transformed M. smegmatis. Methods Different component of Mycobacterium tuberculosis were separated by differential velocity centrifugation, and subcellular location of Rv2629 was examined by Western-Blot. pMV261 plasmid was used to transformed the Rv2629 gene to M. smegmatis. RIF resistant was assayed by BACTEC MGIT 960 instrument. Results The subcellular location of Rv2629 was major at cell wall and cell membrane. The MIC of RIF for M. smegmatis transformed with mutated Rv2629 was 160μg/mL. While the MIC for wild type Rv2629 gene was 20μg/mL similar to the parental strain. Conclution These results indicate that the 191A/C mutation of Rv2629 gene is associated with RIF resistance.

Key words

M. tuberculosis / Rv2629 / Subcellular location / Shuttle plasmid pMV261 / Drug susceptibility testing

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WANG Qing-zhong1,2; ZHANG Lu1; XU Ying1; CHEN Jia-zhen1; ZHU Bing-dong1; QIE Ya-qing1; WANG Jiu-ling1; WANG Hong-hai1. Subcellular location of Rv2629 in M. tuberculosis and its transformed version into M. smegmatis using the pMV261 plasmid[J]. Journal of Microbes and Infections. 2008, 3(2): 90-93