Interferon-β induction and related signaling pathways in human hepatocyte cell lines

CHEN Hui1; WU Min2; YU Shi-Yan1; 2; CHEN Jie-Liang1

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Journal of Microbes and Infections ›› 2009, Vol. 4 ›› Issue (2) : 81-87.

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Interferon-β induction and related signaling pathways in human hepatocyte cell lines

CHEN Hui¹；WU Min²； YU Shi-Yan^1,2； CHEN Jie-Liang^1,2； YUAN Zheng-Hong^1,2

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Abstract

Interferon-β plays an important role in innate immunity against viral infection. Hepatocytes, as hepatitis viruses-harboring cells, are reported to possess the potential to inductively express interferon-β. However, a practical in vitro cell model for investigating the interplay between hepatitis B virus and host cells is rarely reported. Here, we determined the inductive expression of interferon-β by interferon-β agonists〔(Newcastle disease virus, NDV) and poly(I∶C)〕in immortalized primary hepatic cell line, PH5CH8,and hepatocarcinoma cell lines, Huh-7 and HepG2. The data demonstrated that inductive expression of interferon-β in PH5CH8 cells was significantly higher than that in Huh7 or HepG2 cells. In addition, the expression level of key molecules critical for interferon-β induction was investigated to clarify the underlying mechanism. The results showed that the background expression level was fairly low in Huh7 and HepG2 cell lines, compared to that in PH5CH8 cells. It is suggested that PH5CH8 cells possess intact potential to produce interferon-β and reconstitution of a selective interferon-deficient hepatic cell line might be achieved via introduction of related molecules critical for interferon induction.

Key words

Hepatocyte / Interferon-β / Induciton signaling pathway / interferon-βagonists

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CHEN Hui¹；WU Min²； YU Shi-Yan^1,2； CHEN Jie-Liang^1,2； YUAN Zheng-Hong^1,2. Interferon-β induction and related signaling pathways in human hepatocyte cell lines[J]. Journal of Microbes and Infections. 2009, 4(2): 81-87