Detection of Borna disease virus ( BDV) genome in the brain of BDV-inoculated Wistar rats by in situ hybridization

LIU Ai-Ping; LI Yu-Jun; LI Xiao-Guang; SONG Wu-Qi; LI Ai-Mei; ZHOU Shu-Ru; ZHANG Feng-Min

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Journal of Microbes and Infections ›› 2009, Vol. 4 ›› Issue (3) : 152-155.

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Detection of Borna disease virus ( BDV) genome in the brain of BDV-inoculated Wistar rats by in situ hybridization

LIU Ai-Ping；LI Yu-Jun；LI Xiao-Guang；SONG Wu-Qi；LI Ai-Mei；ZHOU Shu-Ru；ZHANG Feng-Min

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Abstract

The aim of the present study was to detect the Borna disease virus ( BDV) genome in the brain of BDV-inoculated Wistar rats by in situ hybridization. The BDV p24 sense probe was labeled with digoxigenin ( DIG) using a DIG RNA labeling kit. The labeling efficiency of the BDV p24 sense probe was conformed by spot test, and the specificity was determined by a dot hybridization assay. The BDV genome was then detected with the probe via in situ hybridization in paraffin brain sections of BDV-inoculated Wistar rats. At the third week post-BDV inoculation, the BDV genome was detected mainly in hippocampus and cortex, with minimal labeling in the thalamus and
hypothalamus. Whereas at the sixth week post-BDV inoculation, the BDV genome was not only detected in hippocampus and cortex, but was also increased in thalamus and hypothalamus, suggesting that the BDV-infected
region was enlarged following prolonged post-inoculation time, and that in situ hybridization with a BDV p24 sense probe can be used to observe the distribution of BDV in the inoculated Wistar rat brain.

Key words

Borna disease virus / Rat / In situ hybridization / Dot hybridization

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LIU Ai-Ping；LI Yu-Jun；LI Xiao-Guang；SONG Wu-Qi；LI Ai-Mei；ZHOU Shu-Ru；ZHANG Feng-Min. Detection of Borna disease virus ( BDV) genome in the brain of BDV-inoculated Wistar rats by in situ hybridization[J]. Journal of Microbes and Infections. 2009, 4(3): 152-155