Genetic polymorphisms of Cryptococcus neoformans isolates from patients infected with human immunodeficiency virus

CHEN Min1; TANG Yong1; PAN Wei-Hua2; LIAO Wan-Qing2

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Journal of Microbes and Infections ›› 2009, Vol. 4 ›› Issue (3) : 156-161.

论著

Genetic polymorphisms of Cryptococcus neoformans isolates from
patients infected with human immunodeficiency virus

CHEN Min¹ ；TANG Yong¹；PAN Wei-Hua²；LIAO Wan-Qing²

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Abstract

The present report was designed to determine the population structure of genotypes and mating type of Cryptococcus neoformans isolates from Chinese patients infected with human immunodeficiency virus( HIV) living in Shanghai. A total of 12 clinical Cryptococcus neoformans isolates were analyzed by internal transcribed spacer ( ITS) sequence using CLUSTAL W1. 83 and MEGA3. 1 software. The phylogenetic tree was statistically tested via
bootstrapping method, and molecular typing was analyzed via polymerase chain reaction ( PCR) -fingerprinting with M13 oligonucleotide as a single primer. Mating type was determined by PCR using two specific primer pairs in
accordance with Chaturvedi et al. The results showed that mating-type α, VNⅠ molecular type ( 9 /12, 75% ) is predominant in clinical Cryptococcus neoformans isolates from this group of patients. Our research provides new
information on the genetic polymorphisms of Cryptococcus neoformans isolates from Shanghai, China.

Key words

Cryptococcus neoformans / Acquired immunodeficiency syndrome / Mating type / Genetic polymorphism

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CHEN Min¹ ；TANG Yong¹；PAN Wei-Hua²；LIAO Wan-Qing². Genetic polymorphisms of Cryptococcus neoformans isolates from
patients infected with human immunodeficiency virus[J]. Journal of Microbes and Infections. 2009, 4(3): 156-161