Approach to syphilis antibodys of stnadurd treated at  previousli untreated and after treatment syphilis change with syphilis serological  tests. Evaluation value of syphilis were used by syphilis serological test detection in the diagnosis of the clinie. Dark field microscopy, RPR, TPPA and TP-IgM-ELISA tests were detected by 135 previously untreated and after completion  treatment of syphilis. 135  previously untreated patients: In 24 cases of primary chancre syphilis,the dark field microscopy  were positive, RPR and TPPA and  TP-IgM-ELISA  of serological test were positive in 83.3%, 91.7% and 91.7% of, respectively. All the serological tests were positive in 93 patients with secondary syphilis. In 18 early latent syphilis, the serological tests were positive 88.9%, 100.0% and 88.9%,respectivedy. 12  months after completion of treatment in RPR and TP-IgM-ELISA test, 20 and 22 primary syphilis were negative in 100.0%. 93 of secondary syphilis were negative in 64.5% and 67.7%, respectively. 18 early latent syphilis were negative in 38.9% in RPR and TP-IgM-ELISA test, respectively. These results reflect the associations of treponemal serological test, when RPR and TP-IgM-ELISA tests is positive, TPPA test is positive well in  previously untreated syphilis,which the TPPA is higher sensitivity method to detect aerly latent syphilis. Twelve months after completion treatment of syphlis , when TP-IgM-ELISA test is negative， RPR test is negative basically. It is indicated that RPR and TPPA test are the best of  serological test of syphilis with clinical  routine diagnosis and obaservation.