Bacterial culture test, the gold standard of diagonosis of Neisseria gonorrhoeae infection, is the most reliable method for diagnosing gonorrhea.. However, very weak resistance of Neisseria gonorrhoeae leads to culture failure. Dection of bacterial morphology only means limited significance for diagnosis of Neisseria gonorrhoeae infection. With the antibiotics resistance in Neisseria gonorrhoeae growing, multi-resistant bacteria have appeared and wildly spreaded. The simple techniques of molecular biology with high sensitivity and high specificity such as single tube multiplex polymerase chain reaction (PCR) have been applicated for laboratory diagnosis of Neisseria gonorrhoeae infection. showing encouraging prospects.