The present paper aims to investigate the clinical effects of combination of metronidazole, clotrimazole and chlorhexidine acetate effervescent tablets and living preparationof lactobacillus on bacterial vaginosis. A total of 100 patients with bacterial vaginosis from the Department of Obstetrics and Gynecology, Hospital of Chinese and Western Integrated Medicine of Jiangsu Province were selected and equally divided into the control group and experimental group. The patients in the control group were only treated with metronidazole, clotrimazole and chlorhexidine acetate effervescent tablets, and the patients in the experimental group were treated with combination of metronidazole, clotrimazole and chlorhexidine acetate effervescent tablets and living preparationof lactobacillus. The curative effects in the two groups were compared after treatment. In the experimental group, 2 cases were lost to follow-up, 35 markedly effective, 8 effective. The markedly effective rate was 72.92% and the total effective rate was 89.58%. In the control group, 1 case was lost to follow-up, 31 markedly effective, 7 effective. The markedly effective rate was 63.27% and the total effective rate was 77.56%. The total effective rate was significantly different in two groups (P<0.05). The recurrence rate in the experimental group was 6.25%, significantly lower than 20.41% in the control group (P<0.05). The results suggest that combination of metronidazole, clotrimazole and chlorhexidine acetate effervescent tablets and living preparation of lactobacillus is safe and effective in treatment of bacterial vaginosis.