The present paper aims to explore drug resistance of 72 non-typhoidal Salmonella isolates and the clinical characteristics of resulting enteritis in children. Stool specimens from 1 665 children experiencing intestinal infection were collected by the Digestive Disorders Ward of Zhongshan Boai Hospital from March 2009 to October 2010. The drug sensitivity testing on 72 non-typhoidal Salmonella isolates was conducted after strain identification and separation. The clinical characteristics of non-typhoidal Salmonella infection were retrospectively analyzed. The results demonstrated that sensitivities of non-typhoidal Salmonella to cefoperazone-tazobactam, Tienam, levofloxacin, piperacillin-tazobactam, and ceftazidine were 100% , 98.61 %, 95.83% , 87.50% and 77.78% , respectively. Antibiotic resistance rate of these non-typhoidal Salmonella to the first and second generation cephalosporins, aminoglycosides , non-enzyme penicillin and cephamycins were all > 80%. Children with intestinal infection by non-typhoidal Salmonella typically presented with clinical characteristics specific to invasive enteritis such as mucous bloody stool , omitting and fever. Older children reported more abdominal pain and showed more abdominal distension and some may deteriorate to non-typhoidal Salmonella sepsis. This study suggests that non-typhoidal Salmonella may have a high sensitivity to carbapenems, enzyme-containing β-lactam compounds, and fluoroquinolones. Therefore , antibiotics should be chosen reasonably and prudently based on drug sensitivity testing as treatment for effectively controlling infection, and preventing the occurrence of further deterioration in patients.