Personnel management plays a key role in the daily management of a biosafety level 3 laboratory (BSL-3). Laboratory personnel typically implement the biosafety management system including rules and regulations, standard operation procedures for pathogenic microbiology. It is these procedures that keep laboratory operations in order and reduce potential safety hazards. Our experiences and lessons learned from BSL-3 laboratory management and additional opinions or considerations on personnel management are summarized here to improve the quality of management in a BSL-3 laboratory.