Typhoid is a global concern. About 21 million people are infected by Salmonella typhi every year, and nearly 5% of them become chronic carriers. Current studies indicate that chronic S. typhi infection is associated with gallstone and gallbladder cancer, a relatively rare neoplasm with increasing incidence rates in certain populations. The incidence rates of gallbladder cancer and gallstone are two-fold in women than in men. The chronic carrier state is a risk factor not only for gallbladder cancer but also for other cancers in some extent. The research on relationships among typhoid carrier state, biofilm and gallstone formation, and gallbladder cancer are reviewed in this paper.