In order to study the disease spectrum and clinical features of Epstein-Barr virus (EBV) infection in children , 260 children with EBV infection diagnosed by serum EBV DNA detection in our hospital were reviewed. The results showed that 60.0% (1 56/260) were under age of 3; angina and fever were the main clinical manifestations of EBV infection , associated with 95.4% and 86.5% of the studied cases respectively. There were 115 cases (44.2%) with ≥10% peripheral blood atypical lymphocytes. 1nfectious mononucleosis (IM) was the typical disease associated with 83.5% (217/260) of the studied cases. Among 43 untypical EBV infections, 32 were upper respiratory tract infection , 4 were hemophagocytic syndrome, 2 were autoimmune hemolytic anemia , and one of each for acute lymphoblastic leukemia accompanied by splenic lymphoma, systemic lupus erythematosus , Letterer-Siwe disease, hand foot and mouth disease and viral rash. 3.2% (7/217) of 1M were severe cases, 18.6% (8/43) of untypical EBV infection were severe, and the difference was significant ( P< 0.05). The prognosis of children with EBV infection was favorable in general. 254 cases were cured, with the cure rate of 97.7%. One patient developed persistent chronic active infection, 2 improved their conditions, 2 were transferred to other faciliti 凶， and 1 died. Severe cases had poor prognosis, especially in those with hemophagocytic syndrome. Therefore, more medical attention should be paid to EBV infection in children.