To screen out the standard clinic strains used for animal model of drug-resistant Mycobacterium tuberculosis (M. tuberculosis), the strains were collected from tuberculosis patients in several provinces of China. The drug susceptibility test with first-line and second-line anti-tuberculosis drugs and drug-resistant gene mutant analysis were performed in 98 clinic isolations, the strains with explicit drug resistant phenotype and mutant were selected for in vivo virulence experiment in mice. Among the 40 strains with explicit drug resistant patterns, the half lethal time (LT₅₀) of 35 strains were shorter than that of H37Rv in the virulence experiment in mice. 18 in 35 strains were rifampin-resistant and isoniazid-resistant. 5 strains were rifampin-resistant, 7 strains were isoniazid-resistant, and 5 strains were sensitive to both isoniazid and rifampin. Standard strain with moderate virulence and explicite genotype was screened out for each drug resistant phenotype. For rifampin-resistance combined with isoniazid-resistance, one strain with LT₅₀≤7 d was selected as its standard strong virulence. For rifampin-resistance or isoniazid-resistance, one strain with LT₅₀≤7 d was selected as its standard strong virulence strain respectively. Each standard strain can be used as candidate standard strain used in M. tuberculosis infection model of guinea pig or other animal.