Metallo-β-lactamase (MBL) can hydrolyze a variety of antibiotics, and is insensitive to conventional β-lactamase inhibitors, such as clavulanic acid and sulbactam. After the reporting of New Delh imetallo-β- lactamase (NDM) ,Enterobacteriaceae producing MBL have been recognized as clinical multidrug-resistant pathogens,and the infectìons caused by them in clinic are becoming increasingly serious. Rapid and accurate detection of this group of strains is an important link to prevent and control the occurrence and spread of infection effectively. The research progress on MBL produced by Enterobacteriaceae covering its discovery, classification, spreading and  detection methods is reviewed in the present paper.